Expression of Blattella germanica allergen 2 gene in E.coli and Production of Its Polyclonal Antibody

Blattella germanica allergen 2(Bla g 2) gene was amplified by RT-PCR from total RNA of Blattella germanica and inserted into pET-41b vector properly. GST-Bla g 2 was expressed in E.coli via IPTG induction, a relative molecular mass of approximately 74kDa was showed by SDS-PAGE. The expressed fusion protein was purified by Ni+-agarose affinity chromatography, an emulsion was prepared with the purified Bla g 2 and injected into rabbits. The titer of antiserum identified by indirect ELISA reached 1:300000. Moreover, antiserum reacted specifically with purified fusion protein and the extract of Blattella germanica. Preparation of Bla g 2 and its polyclonal antibody offer help to the dialysis and therapy of patients who are allergic to Blattella germanica.